Bioinformatics Tools

Design gRNAs, analyse seed-based off-targets, and verify target sites with PAM-aware matching.

PCR / RT-qPCR / inverse / nested primer design with SantaLucia nearest-neighbour thermodynamics and secondary-structure checks.

Extract promoter regions, detect core elements, predict TF-binding sites and identify candidate transcription factors.

HWE, allele frequency, case–control association, odds ratios, FDR & Bonferroni correction, Manhattan data prep.

Align multiple sequences against a reference, call SNPs and indels, build an IUPAC consensus, compute pairwise distances.

Restriction cloning, Gibson assembly, Golden Gate, TA cloning, restriction mapping, ORF & feature annotation.

Simulate DNA electrophoresis — choose agarose %, voltage, buffer and stain; get a real rendered gel image with a DNA ladder.

Simulate SDS-PAGE, Native-PAGE, Tricine-SDS and gradient gels. Computes MW, pI, GRAVY from protein sequences and renders the gel.

Calculate Rf values from measured distances or predicted references, view a virtual chromatogram, and cross-check against a compound Rf database.

Predict TLC / paper chromatography Rf directly from chemical structure (SMILES) using logP, TPSA, HBD and aromaticity — with solvent & chamber type.

Contamination monitoring, callus induction assessment, regeneration efficiency and root/shoot growth analysis with charts.