CRISPR gRNA Design
Ready
Design gRNAs, analyse seed-based off-targets, and verify target sites with PAM-aware matching.
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GWAS Bioinformatics
Ready
HWE, allele frequency, case–control association, odds ratios, FDR & Bonferroni correction, Manhattan data prep.
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Plasmid / Construct Design
Ready
Restriction cloning, Gibson assembly, Golden Gate, TA cloning, restriction mapping, ORF & feature annotation.
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Primer Design Suite
Ready
PCR / RT-qPCR / inverse / nested primer design with SantaLucia nearest-neighbour thermodynamics and secondary structure checks.
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SNP / Indel Analysis
Ready
Align multiple sequences against a reference, call SNPs and indels, build an IUPAC consensus, compute pairwise distances.
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